Real-time visualization of burn severity and hypoxic response is important in understanding wound healing outcomes. Herein we propose a novel approach to macroscopically image burn induced hypoxia through endogenous PpIX delayed fluorescence. The approach is validated in-vivo using athymic nude mice with induced full-thickness burns. PpIX delayed fluorescence imaging was performed longitudinally for 3 days post injury. The localization of PpIX signals was also correlated to Indocyanine Green fluorescence imaging, a technique for blood perfusion and tissue injury in the burn wound. The current results highlight the capability of the approach for real-time in-vivo quantification of hypoxic response to burn wounds.
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