STimulated Emission Depletion (STED) microscopy enables imaging of biological samples combining significantly
improved optical resolution with all benefits of confocal microscopy. Especially, by combining multi-channel image
acquisition with high spatial resolution opens up a new understanding of co-localization experiments on nanoscales.
Such a microscope provides new insights in various fields of biology, such as cell and membrane biology, neurobiology
and physiology. We present new developments and a variety of biological examples for STED microscopy, showing
structural details on scales well below 70nm and give an overview of possible field of applications, mainly focused on live cell imaging.
STED microscopy enables confocal imaging of biological samples with a resolution that is not limited by diffraction. It
provides new insights in various fields of biology, such as membrane biology, neurobiology and physiology. Its three
dimensional sectioning ability allows the acquisition of high resolution image stacks. Furthermore, STED microscopy
enables the recording of dynamic processes and live cell images. We present two-color imaging in confocal STED
microscopy with a single STED wavelength. Pulsed and continuous wave lasers in the visible and near infra-red
wavelengths range are used for stimulated emission. The resolution enhancement is demonstrated in comparison to
confocal imaging with biological specimens.
STED microscopy has gained recognition as a method to break the diffraction limit of conventional light microscopy.
Despite being a new technique, STED is already successfully implemented in life science research. The resolution
enhancement is achieved by depleting fluorescent markers via stimulated emission. The performance is significantly
dependent on the laser source and the fluorescence markers. Therefore the use of novel fluorescent markers in
conjunction with the right laser system was the main focus of our research. We present new developments and
applications of STED microscopy, unraveling structural details on scales below 90nm and give an overview of required
specifications for the solid state laser systems.
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