KEYWORDS: Quantum dots, Multiplexing, Atmospheric particles, Short wave infrared radiation, Quantum efficiency, Infrared radiation, Infrared imaging, In vivo imaging, Signal to noise ratio, Shortwaves
Fluorescence bioimaging utilizing the near infrared-II (NIR-II; 1000 – 1700 nm) window, also named shortwave infrared (SWIR) imaging, exhibits superior penetration depth and signal-to-noise ratio compared to NIR-I imaging. We synthesized and characterized multiple lead sulfide/cadmium sulfide (PbS/CdS) core/shell quantum dots (QDs) with distinct, monodisperse size distributions, narrow emission, high quantum yield, and photostability for use in multiplexed NIR-II imaging. Following micelle encapsulation, the QDs are water-soluble, stably dispersed, and functionalized for easy click chemistry conjugation with targeting moieties. In vivo mice imaging with IV injected QDs showed very strong and distinctive signals for multiplexed imaging.
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