Prof. Yuval Garini Profile

Prof. Yuval Garini

Professor & Head of Nanotechnology Institute at Bar-Ilan Univ

SPIE Involvement:

Conference Program Committee | Author

Publications (23)

SPIE Journal Paper | 9 September 2023 Open Access

Compressive hyperspectral microscopy for cancer detection

Yaniv Oiknine, Marwan Abuleil, Eugene Brozgol, Isaac August, Iris Barshack, Ibrahim Abdulhalim, Yuval Garini, Adrian Stern

JBO, Vol. 28, Issue 09, 096502, (September 2023) https://doi.org/10.1117/12.10.1117/1.JBO.28.9.096502

KEYWORDS: Microscopes, Microscopy, Light sources and illumination, Cancer detection, Imaging spectroscopy, Imaging systems, Biopsy, Optical filters, Tunable filters, Medical image reconstruction

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SignificanceHyperspectral microscopy grants the ability to characterize unique properties of tissues based on their spectral fingerprint. The ability to label and measure multiple molecular probes simultaneously provides pathologists and oncologists with a powerful tool to enhance accurate diagnostic and prognostic decisions. As the pathological workload grows, having an objective tool that provides companion diagnostics is of immense importance. Therefore, fast whole-slide spectral imaging systems are of immense importance for automated cancer prognostics that meet current and future needs.AimWe aim to develop a fast and accurate hyperspectral microscopy system that can be easily integrated with existing microscopes and provide flexibility for optimizing measurement time versus spectral resolution.ApproachThe method employs compressive sensing (CS) and a spectrally encoded illumination device integrated into the illumination path of a standard microscope. The spectral encoding is obtained using a compact liquid crystal cell that is operated in a fast mode. It provides time-efficient measurements of the spectral information, is modular and versatile, and can also be used for other applications that require rapid acquisition of hyperspectral images.ResultsWe demonstrated the acquisition of breast cancer biopsies hyperspectral data of the whole camera area within ∼1 s. This means that a typical 1 × 1 cm2 biopsy can be measured in ∼10 min. The hyperspectral images with 250 spectral bands are reconstructed from 47 spectrally encoded images in the spectral range of 450 to 700 nm.ConclusionsCS hyperspectral microscopy was successfully demonstrated on a common lab microscope for measuring biopsies stained with the most common stains, such as hematoxylin and eosin. The high spectral resolution demonstrated here in a rather short time indicates the ability to use it further for coping with the highly demanding needs of pathological diagnostics, both for cancer diagnostics and prognostics.

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Proceedings Article | 16 February 2017 Paper

Novel system for measuring giant spectral images and its application for cancer detection

Pramod Kumar, Irina Marin, Daniela Necula, Irena Bronshtein-Berger, Moshe Linder, Zav Shotan, Gali Zimmerman-Moreno, Arnaldo Mayer, Iris Barshack, Yuval Garini

Proceedings Volume 10068, 100680N (2017) https://doi.org/10.1117/12.2253531

KEYWORDS: Cancer, Imaging spectroscopy, Diagnostics, Statistical analysis, Sensors, Imaging systems, Pathology, Forensic science, Tissues, Microscopy, Sagnac interferometers, Cameras, RGB color model, Interferometers, Raman spectroscopy, Spectroscopy

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Proceedings Article | 3 November 2011 Paper

Novel approaches for near and far field super-resolved imaging

Zeev Zalevsky, Aviram Gur, Ran Aharoni, Vladimir Kutchoukov, Yuval Garini, Yevgeny Beiderman, Vicente Micó, Javier García

Proceedings Volume 8011, 80116M (2011) https://doi.org/10.1117/12.899573

KEYWORDS: Glucose, Blood, Microscopes, Speckle pattern, Skin, Tissue optics, Imaging systems, Cameras, Sensors, Spectroscopy

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SPIE Journal Paper | 1 July 2009

Tethered particle motion mediated by scattering from gold nanoparticles and darkfield microscopy

Heidelinde Dietrich, Bernd Rieger, Frank Wiertz, Frederik de Groote, Hendrik Heering, Ian Young, Yuval Garini

JNP, Vol. 3, Issue 01, 031795, (July 2009) https://doi.org/10.1117/12.10.1117/1.3174445

KEYWORDS: Particles, Gold, Microscopy, Nanoparticles, Light scattering, Scattering, Signal to noise ratio, Molecules, Motion measurement, Metals

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SPIE Journal Paper | 1 October 2007

Structured illumination microscopy using extraordinary transmission through sub-wavelength hole-arrays

Margreet Docter, Peter van den Berg, Paul Alkemade, Vladimir Kutchoukov, Oana Piciu, André Bossche, Ian Young, Yuval Garini

JNP, Vol. 1, Issue 01, 011665, (October 2007) https://doi.org/10.1117/12.10.1117/1.2794786

KEYWORDS: Microscopes, Confocal microscopy, Point spread functions, Microscopy, Optical transfer functions, Charge-coupled devices, Image resolution, Gold, Near field scanning optical microscopy, Structured light

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Proceedings Article | 10 February 2007 Paper

A new optical method for characterizing single molecule interactions based on dark field microscopy

Heidelinde Dietrich, Bart Vermolen, Bernd Rieger, Ian Young, Yuval Garini

Proceedings Volume 6444, 644403 (2007) https://doi.org/10.1117/12.699040

KEYWORDS: Molecules, Gold, Molecular interactions, Microscopy, Proteins, Light scattering, Particles, Analytical research, Fluorescence resonance energy transfer, Fluorescence correlation spectroscopy

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Proceedings Article | 20 April 2006 Paper

Measuring the near-field of extraordinary transmission through a periodic hole-array

M. Docter, I. Young, O. Piciu, A. Bossche, P. F. Alkemade, P. van den Berg, Y. Garini

Proceedings Volume 6195, 61951L (2006) https://doi.org/10.1117/12.661969

KEYWORDS: Near field, Molecules, Microscopes, Surface plasmons, 3D modeling, Polarization, Luminescence, Water, Metals, Diffraction

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Proceedings Article | 27 February 2006 Paper

Advances in the development of a novel method to be used in proteomics using gold nanobeads

Heidelinde R. Dietrich, Ian Young, Yuval Garini

Proceedings Volume 6092, 60920C (2006) https://doi.org/10.1117/12.645908

KEYWORDS: Gold, Proteins, Molecules, Diffusion, Light scattering, Analytical research, Cell mechanics, Molecular interactions, Microscopy, Biopolymers

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Proceedings Article | 21 February 2006 Paper

Absolute fluorescence calibration

I. Young, Y. Garini, B. Vermolen, G. Liqui Lung, G. Brouwer, S. Hendrichs, M. el Morabit, J. Spoelstra, E. Wilhelm, M. Zaal

Proceedings Volume 6088, 60880U (2006) https://doi.org/10.1117/12.638559

KEYWORDS: Light emitting diodes, Calibration, Microscopes, Imaging systems, Luminescence, Photons, Cameras, Light sources, Electrons, Rhodamine

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Proceedings Article | 31 March 2005 Paper

A novel concept for a mid-field microscope

Margreet Docter, I. Young, Vladimir Kutchoukov, Andre Bossche, Paul Alkemade, Yuval Garini

Proceedings Volume 5703, (2005) https://doi.org/10.1117/12.588216

KEYWORDS: Microscopes, Surface plasmons, Diffraction, Collimation, Near field, Near field scanning optical microscopy, Light, Glasses, Spatial resolution, Molecules

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Proceedings Article | 29 March 2005 Paper

Adaptation of nanoarrays for the study of alpha-synuclein aggregation: preliminary results

Heidelinde Dietrich, Richard van den Doel, Wolfgang Hoyer, Wim van Oel, Guus Liqui Lung, Yuval Garini, Thomas Jovin, Ian Young

Proceedings Volume 5699, (2005) https://doi.org/10.1117/12.587493

KEYWORDS: Humidity, Proteins, Temperature metrology, Microscopes, CCD cameras, Luminescence, Liquids, Calibration, Signal detection, Objectives

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Proceedings Article | 29 March 2005 Paper

Three-dimensional analysis tool for segmenting and measuring the structure of telomeres in mammalian nuclei

Bart Vermolen, Ian Young, Alice Chuang, Landon Wark, Tony Chuang, Sabine Mai, Yuval Garini

Proceedings Volume 5699, (2005) https://doi.org/10.1117/12.590232

KEYWORDS: Image segmentation, 3D image processing, Luminescence, Deconvolution, Image analysis, Spherical lenses, Microscopy, Digital imaging, Quantitative analysis, 3D acquisition

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Proceedings Article | 23 March 2005 Paper

Gold nanoparticles: a novel application of spectral imaging in proteomics - preliminary results

Heidelinde Dietrich, Ian Young, Yuval Garini

Proceedings Volume 5694, (2005) https://doi.org/10.1117/12.587517

KEYWORDS: Gold, Particles, Proteins, Light scattering, Imaging spectroscopy, Nanoparticles, Metals, Charge-coupled devices, CCD cameras, Image analysis

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Proceedings Article | 22 January 2005 Paper

Optical detection of electrokinetically manipulated single molecules in a nanofluidic chip

Gea Parikesit, Vladimir Kutchoukov, Andre Bossche, Ian Young, Yuval Garini

Proceedings Volume 5718, (2005) https://doi.org/10.1117/12.583566

KEYWORDS: Molecules, Dielectrophoresis, Particles, Microfluidics, Electrodes, Spectral resolution, Liquids, Interfaces, Microscopes, Confocal microscopy

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Proceedings Article | 8 October 2004 Paper

Optical detection of single molecules in nanofluidic chips

Gea Parikesit, Vladimir Kutchoukov, Wim van Oel, Guus Lung, Andre Bossche, Ian Young, Yuval Garini

Proceedings Volume 5515, (2004) https://doi.org/10.1117/12.559386

KEYWORDS: Particles, Liquids, Molecules, Electrodes, Dielectrophoresis, Interfaces, Aluminum, Luminescence, Singular optics, Nanolithography

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Proceedings Article | 13 July 2004 Paper

LEDs for fluorescence microscopy

Ian Young, Yuval Garini, Heidi Dietrich, Wim van Oel, Guus Liqui Lung

Proceedings Volume 5324, (2004) https://doi.org/10.1117/12.525932

KEYWORDS: Light emitting diodes, Lamps, Mercury, Luminescence, Microscopy, Light sources, Microscopes, Xenon, Modulation, Optical filters

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Proceedings Article | 16 June 2004 Paper

Toward the development of a three-dimensional mid-field microscope

Yuval Garini, Vladimir Kutchoukov, Andre Bossche, Paul Alkemade, Margreet Docter, Piet Verbeek, Lucas van Vliet, Ian Young

Proceedings Volume 5327, (2004) https://doi.org/10.1117/12.537623

KEYWORDS: Plasmons, Metals, Microscopes, Surface plasmons, Microscopy, Dispersion, Diffraction, Near field, Photons, Near field optics

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Proceedings Article | 9 June 1998 Paper

New compact-design interferometer-based spectral imaging system for biomedical applications

Mosik Lavi, Uri Milman, Dario Cabib, Yuval Garini, Amir Gil, Ton Juta, Michael Adel

Proceedings Volume 3261, (1998) https://doi.org/10.1117/12.310567

KEYWORDS: Imaging spectroscopy, Interferometers, Imaging systems, Spectral resolution, Sagnac interferometers, Image enhancement, Optical components, Charge-coupled devices, Interferometry, Remote sensing

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Proceedings Article | 10 May 1996 Paper

Microscopic and mesoscopic spectral bioimaging

Daniel Farkas, Byron Ballou, Gregory Fisher, Daniel Fishman, Yuval Garini, Wenhua Niu, Elliot Wachman

Proceedings Volume 2678, (1996) https://doi.org/10.1117/12.239508

KEYWORDS: Microscopy, Luminescence, Imaging spectroscopy, Tumors, In vivo imaging, Skin, Tissues, Acousto-optics, Tissue optics, Image resolution

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Proceedings Article | 10 May 1996 Paper

Multicolor FISH using a novel spectral bioimaging system

Dirk Soenksen, Yuval Garini, Irit Bar-Am

Proceedings Volume 2678, (1996) https://doi.org/10.1117/12.239550

KEYWORDS: Imaging systems, Luminescence, Image filtering, Optical filters, Interferometers, Imaging spectroscopy, Genetics, Image classification, Fourier spectroscopy, Charge-coupled devices

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Proceedings Article | 10 May 1996 Paper

Spatially resolved Fourier transform spectroscopy (spectral imaging): a powerful tool for quantitative analytical microscopy

Dario Cabib, Robert Buckwald, Yuval Garini, Dirk Soenksen

Proceedings Volume 2678, (1996) https://doi.org/10.1117/12.239547

KEYWORDS: Imaging spectroscopy, Charge-coupled devices, Interferometers, Fourier transforms, Spectroscopy, Imaging systems, Microscopes, Luminescence, Spectral resolution, Fourier spectroscopy

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Proceedings Article | 5 April 1996 Paper

Novel spectral bioimaging system as an imaging oximeter in intact rat brain

Dirk Soenksen, Thomas Sick, Yuval Garini

Proceedings Volume 2679, (1996) https://doi.org/10.1117/12.237587

KEYWORDS: Absorption, Brain, Tissues, Imaging systems, Nitrogen, Imaging spectroscopy, Spectroscopy, Neuroimaging, Tissue optics, Reflection

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Proceedings Article | 1 February 1995 Paper

Novel spectral imaging system combining spectroscopy with imaging applications for biology

Zvi Malik, Dario Cabib, Robert Buckwald, Yuval Garini, Dirk Soenksen

Proceedings Volume 2329, (1995) https://doi.org/10.1117/12.200882

KEYWORDS: Imaging spectroscopy, Imaging systems, Spectroscopy, Luminescence, Biology, Image analysis, Associative arrays, Biological research, Blood, Microscopy

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