Prof. Jun Zheng Profile

Prof. Jun Zheng

at Zhejiang Univ

SPIE Involvement:

Author

Publications (6)

SPIE Journal Paper | 15 March 2024 Open Access

3D polarization-interference holographic histology for wavelet-based differentiation of the polycrystalline component of biological tissues with different necrotic states. Forensic applications

Alexander Ushenko, Alexander Dubolazov, Jun Zheng, Alexandra Litvinenko, Mykhaylo Gorsky, Yuriy Ushenko, Iryna Soltys, Olexander Salega, Zhebo Chen, Oleh Wanchuliak

JBO, Vol. 29, Issue 05, 052920, (March 2024) https://doi.org/10.1117/12.10.1117/1.JBO.29.5.052920

KEYWORDS: Polarization, Wavelets, Tissues, Holography, Biological samples, Lung, Myocardium, Tissue optics, Statistical analysis, Biological research

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SignificanceThe interference-holographic method of phase scanning of fields of scattered laser radiation is proposed. The effectiveness of this method for the selection of variously dispersed components is demonstrated. This method made it possible to obtain polarization maps of biological tissues at a high level of depolarized background. The scale-selective analysis of such maps was used to determine necrotic changes in the optically anisotropic architectonics of biological tissues.ObjectiveDevelopment and experimental approbation of layered phase polarimetry of repeatedly scattered fields in diffuse layers of biological tissues. Application of scale-selective processing of the found coordinate distributions of polarization states in various phase sections of object fields. Determination of criteria (markers) for histological differential diagnosis of the causes of necrotic changes in optical anisotropy of biological tissues.ApproachWe used a synthesis of three instrumental and analytical methods. Polarization-interference registration of laser radiation scattered by a sample of biological tissue. Digital holographic reconstruction and layered phase scanning of distributions of complex amplitudes of the object field. Analytical determination of polarization maps of various phase cross-sections of repeatedly scattered radiation. Application of wavelet analysis of the distributions of polarization states in the phase plane of a single scattered component of an object field. Determination of criteria (markers) for differential diagnosis of necrotic changes in biological tissues with different morphological structure. Two cases are considered. The first case is the myocardium of those who died as a result of coronary heart disease and acute coronary insufficiency. The second case is lung tissue samples of deceased with bronchial asthma and fibrosis.ResultsA method of polarization-interference mapping of diffuse object fields of biological tissues has been developed and experimentally implemented. With the help of digital holographic reconstruction of the distributions of complex amplitudes, polarization maps in various phase sections of a diffuse object field are found. The wavelet analysis of azimuth and ellipticity distributions of polarization in the phase plane of a single scattered component of laser radiation is used. Scenarios for changing the amplitude of the wavelet coefficients for different scales of the scanning salt-like MHAT function are determined. Statistical moments of the first to fourth orders are determined for the distributions of the amplitudes of the wavelet coefficients of the azimuth maps and the ellipticity of polarization. As a result, diagnostic markers of necrotic changes in the myocardium and lung tissue were determined. The statistical criteria found are the basis for determining the accuracy of their differential diagnosis of various necrotic states of biological tissues.ConclusionsNecrotic changes caused by “coronary artery disease–acute coronary insufficiency” and “asthma–pulmonary fibrosis” were demonstrated by the method of wavelet differentiation with polarization interference with excellent accuracy.

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Proceedings Article | 5 January 2024 Paper

Polarization-holographic phasometry of the layered vector structure of laser object fields of soft matter polycrystalline layers

Alexander Dubolazov, Alexander Ushenko, Igor Panko, Valeriy Sklyarchuk, Yaroslav Struk, Ivan Mikirin, Jun Zheng, V. Tymchuk

Proceedings Volume 12938, 1293820 (2024) https://doi.org/10.1117/12.3014689

KEYWORDS: Polarization, Digital holography, Tissue optics, Holography, Tissues, Polarimetry, Biomedical optics, Laser optics, Laser radiation, Anisotropy

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