A method of azimuthally invariant 3d Mueller-matrix mapping of the distributions of the parameters of phase and amplitude anisotropy of partially depolarizing layers of high-quality (group 1 - high density) and low-quality (group 2 - low density) polyethylene polymer films has been proposed and substantiated. layer-by-layer coordinate distributions of the magnitude of the set of Mueller-matrix invariants (MMI) of polymer films of both types were obtained in the volume of polymer samples.
The method of stock-polarimetry with spatial-frequency filtering of the distributions of the Muller-matrix invariants (MMI) for analytically differentiating the manifestations of the phase anisotropy mechanisms of different-scale fibrillar networks of histological sections of biological tissues of different morphological structure and physiological state is analytically substantiated. It has been established that all statistical moments of the first and fourth orders characterizing the distribution of the values of the matrix element of the linear birefringence of the large-scale fibrillar optically anisotropic component of benign and malignant tumors are diagnostically sensitive. The following levels of balanced accuracy have been achieved: 77,8% Ri=3;4 (good quality diagnostic test), Ri=1;2 = 91,7% 94,4% (excellent quality diagnostic test). For the first time, a polarization reconstruction method with spatial-frequency differentiation of the distributions of MMI values characterizing circular birefringence of histological sections of multilayer biological tissues was developed to differentiate changes in the phase anisotropy of polycrystalline networks in the early stages of oncological pathology - precancerous conditions (simple atrophy, polyp) of the rectal wall. On this basis, a high level of balanced accuracy R ~ 85% of the diagnostic test for differentiating the manifestations of the optical activity of endometrial networks in a precancerous state has been achieved.
The use of physically sound and analytically determined algorithms for reconstructing parameters characterizing linear birefringence and dichroism of networks of biological crystals in differentiating changes in optical anisotropy associated with different degrees of severity of pathology - precancerous (atrophy and polyp endometrium) conditions of the cervix. Development and testing of the ―two-wave‖ method of the Muller-matrix reconstruction of parameter values characterizing the phase and amplitude anisotropy of polycrystalline films of bile and blood plasma for the diagnosis of systemic (type II diabetes) and oncological (breast cancer) diseases.
The 3D Muller-matrix mapping of map depolarization depolarizing layers of biological tissues of different morphological structure and physiological state is proposed and substantiated. 3D maps of depolarization of optically thick histological sections of myocardium tissue with spatially structured optically anisotropic fibrillary network, as well as parenchymatous rat liver tissue with “islet” polycrystalline structure were obtained. The dependences of the magnitude of the statistical moments of the 1st-4th order, which characterize the layered distributions of the degree of depolarization of different types of biological tissues, are determined. The study of the possibilities of differentiation of benign (adenoma) and malignant (carcinoma) changes of histological sections of the tissue of the uterine tissue biopsy was conducted. In addition, the possibility of diagnosing the degree of blood loss in the liver was investigated. The dependences of the magnitude of the statistical moments of the first and fourth orders, which characterize layer-by-layer distributions of the values of the degree of depolarization of various types of biological tissues are determined. The possibility of differentiation of benign (adenoma) and malignant (carcinoma) changes in the histological sections of the biopsy of the uterus wall tissue was studied. In addition, the possibility of diagnosing the degree of hepatic blood loss was investigated.
This work is aimed at generalizing the methods of laser polarimetry in the case of depolarizing optically anisotropic biological layers. A method of differential polarization mapping for reproducing Mueller-matrix images of fluctuations of linear and circular birefringence and dichroism of diffuse layers of biological tissues of various morphological structures is proposed and substantiated. The coordinate distributions of the elements of a second-order differential matrix of histological sections of brain tissue with a spatially structured optically anisotropic fibrillar network and parenchymal tissue of the rectum wall with an "island" polycrystalline structure were determined. Within the framework of the statistical analysis of polarization-reproduced Müller-matrix images of phase and amplitude anisotropy fluctuations, a significant sensitivity of statistical moments of the third and fourth orders to the changes in the polycrystalline structure of the depolarizing layers of biological tissues was observed.
An optical model of the polycrystalline structure of the human blood film is proposed as a superposition of completely polarized and depolarized components. Analytical algorithms for describing the polarization manifestations of such components are found. A new technique for laser sounding of blood films and detection of polarization-inhomogeneous fields by means of variations in the states of polarization of the reference wave is developed. The algorithm of digital holographic reconstruction of distributions of complex amplitudes of the polarization-inhomogeneous object field of polycrystalline films of blood is used. Layered maps of the distribution of azimuth and ellipticity of polarization of the object field of polycrystalline films of blood were obtained and analyzed. 3D distributions of the linear and circular birefringence and dichroism of such films are determined. Sensitivity, specificity and balanced accuracy of the method of digital polarization-holographic 3D reconstruction of the polycrystalline structure of blood films are determined. Statistical analysis of polarization maps of the polycrystalline structure of blood films revealed objective criteria for the diagnosis of breast cancer.
The method of azimuthally invariant 3D Muller-matrix mapping of distributions of phase and amplitude anisotropy parameters of partially depolarizing layers of biological tissues of different morphological structures is proposed and substantiated. In the volume of biological samples, the coordinate distributions of the magnitude of the set of Mullermatrix invariants (MMI) histological sections of the myocardium tissue with a spatially structured optical anisotropic fibrillary network, as well as parenchymal tissue of the rat liver with an islet polycrystalline structure, were obtained. The "phase" dependences of the magnitude of the statistical moments of the 1 st - 4 th orders, which characterize the distributions of the MMI values of polarization manifestations of the parameters of linear and circular birefringence and dichroism of the polycrystalline component of different types of biological tissues, are determined. A comparative study was made of the possibilities of differentiation of changes in the parameters of optical anisotropy using traditional 2D and 3D Muller-matrix mapping methods. The optimal conditions for the differentiation of polycrystalline structures of biological tissues - the range of phase sections and the most sensitive parameters - are the statistical moments of the 3rd and 4th orders that characterize the distributions of MMI associated with the polarization manifestations of linear birefringence and dichroism of different types of optically anisotropic structures.
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